Composite

Part:BBa_K3002125:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-21)


Level 1 – Wildtype-PETase + ARS + sp20-His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1075
    Illegal PstI site found at 2048
    Illegal NgoMIV site found at 810
    Illegal NgoMIV site found at 837
    Illegal NgoMIV site found at 2539
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.



Source

This part is a combination of the parts BBa_K3002027 (PAR-promoter), BBa_K3002006 (RPL23-Terminator), BBa_K3002028 (CDS of Wildtype-PETase), BBa_K3002009 (ARS), BBa_K3002018 (spSP20-His-tag), BBa_K3002302 (connector B1-B2), BBa_K3002303 (connector B2-B3), BBa_K3002304 (connector B4-B5) and BBa_K3002305 (connector B5-B6).

References